Dr. Dharmalingam Kirubakaran’s research focuses on the green synthesis of nanoparticles and their multifaceted biomedical applications, encompassing antioxidant, antibacterial, anti-inflammatory, antidiabetic, and anticancer activities. His work extensively explores plant-mediated nanoparticle synthesis using bioactive extracts from species such as Strobilanthes cordifolia, Acmella caulirhiza, Cajanus albicans, Lantana camara, and Mangifera indica, combining traditional phytochemistry with advanced nanotechnology approaches. Characterization techniques employed include UV-Visible spectrophotometry, Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), Scanning and Transmission Electron Microscopy (SEM/TEM), Energy Dispersive Spectroscopy (EDAX), and Dynamic Light Scattering (DLS), ensuring comprehensive analysis of particle morphology, size, and stability. Complementing synthesis studies, his in vitro assessments encompass antibacterial activity against Gram-positive and Gram-negative bacteria, antioxidant assays such as DPPH, ABTS, H₂O₂, and FRAP, anti-inflammatory and antidiabetic evaluations, anticholinergic enzyme inhibition, and anticancer effects on multiple cell lines including MCF-7, A431, HeLa, and A549. Additional investigations include mosquito larvicidal bioassays, photocatalytic dye degradation, and ethnobotanical studies of medicinal plants. His publications reveal a strong emphasis on sustainable, plant-based nanomaterials for biomedical applications, with work on PVA–PEG and chitosan-based nanocomposites highlighting potential in food packaging, therapeutic interventions, and drug delivery. Collectively, his research integrates phytochemistry, nanotechnology, and pharmacological evaluation to advance environmentally friendly and biologically effective solutions for healthcare and environmental challenges.