👨🏫 Dr. Alessandra Amendola an Dr at N.L.Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani Italy and Research, Department of Immunology and Microbiology , stands as a distinguished academic and researcher in the domain of Virologia. Holding a Ph.D on Cellular and Molecular Biology, Dep. of Biology, University of Rome “Tor Vergata”, Italy.their professional journey exemplifies dedication and expertise. 📚
🌐 Professional Profiles:
🎓Education:
1992: Degree in Biology with Honours, University of Rome “Tor Vergata“, Italy.1992-95: Ph.D. in Cellular and Molecular Biology, Dep. of Biology, University of Rome “Tor Vergata”, Italy. 2002: Postgraduate studies in Microbiology and Virology, Dept. of Medicine, University of Rome “Tor Vergata”, Italy.
🌍Current Assignments:
Contact person for ISO 9001:2015 Quality Certification, Laboratory of Virology, since 2018. Member of the Regional Order of Biologists of Lazio and Abruzzo, 2022-2026. Member for Healthcare Management in the Control Commission of the Italian Association of Healthcare Managers ANAAO-ASSOMED, since October 2023.
💼Skills and Experience:
Qualitative and Quantitative molecular diagnostics for viral infections (PCR, RT-PCR, TMA, bDNA, Real-time PCR, NASBA). Serological diagnostics for viral infections (ELISA, immunofluorescence, serum neutralisation, Immunoblot). Cell cultures, including primary and various cell lines. Virus isolation, cultivation, and determination of infectious titre in biocontainment laboratories. Flow cytofluorimetry, nucleic acid handling, purification of proteins, nucleic acid electrophoresis.
🔍Research Experiences:
Pathogenesis of viral infections including HIV and SARS-CoV-2. Molecular mechanisms involved in HIV infection, apoptosis, and senescence. Diagnostic methods development and analysis for viral diseases.Cell cultures, immunohistochemistry, flow cytometry, immunoprecipitation.
📚Top Noted Publication:
2019-novel coronavirus severe adult respiratory distress syndrome in two cases in Italy: an uncommon radiological presentation Published on 2020/4/1Cited by 210
Extracellular ATP acts on P2Y2 purinergic receptors to facilitate HIV-1 infection Published on 2011/8/29 Cited by 200
Sequential involvement of Cdk1, mTOR and p53 in apoptosis induced by the HIV-1 envelope Published on 2002/8/1 Cited by 183
Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/Fkbp12-Rapamycin–associated protein–mediated P53 …Published on 2001/10/15 Cited by 180
Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis Published on 1997/10/1 Cited by 160
Induction of” tissue” transglutaminase in HIV pathogenesis: evidence for high rate of apoptosis of CD4+ T lymphocytes and accessory cells in lymphoid tissues. Published on 1996/10/1 Cited by 149
Nerve growth factor is an autocrine factor essential for the survival of macrophages infected with HIV Published on1999/11/23 Cited by146
Inhibition of “tissue” transglutaminase increases cell survival by preventing apoptosis Published on1999/11/26 Cited by 137