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 Dr. Pushpendra D Pratap |Cervical Cancer| Best Researcher Award

 

Completed my Ph.D. in Biochemistry under the supervision of Prof. Syed Tasleem Raza, Professor, Department of Biochemistry, and Controller of Examination (COE), from ERA University. I have a profound ability to comprehend complex technologies in biochemistry, genomics, proteomics, and more.

Profile

  1. Orcid

 

  1. Scholar

🔬 Current Research

I am currently working on:

  • Single nucleotide gene polymorphisms (SNPs) of interleukins (ILs) and their association with cervical cancer susceptibility.
  • Evaluation of interleukins and other markers’ levels through ELISA.
  • Human papillomavirus (HPV) analysis through RT-PCR, a recognized causative agent for cervical cancer and other carcinomas.
  • Diagnostic tests for HLA-B-27 and MDTB through RT-PCR.
  • COVID-19 testing through RT-PCR.

Additionally, I have experience in the molecular biology of various cancers and other diseases, focusing on signaling pathways, angiogenesis, cell proliferation, apoptosis, autophagy, inflammation, oxidative stress, and targeted therapeutic interventions.

🧪 Expertise

  • Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)
  • Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  • Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
  • KingFisher Duo Prime Extraction System & QS 5Dx qPCR Machine and Analysis Software

🎓 Professional Qualification

  • Ph.D. in Biochemistry (2024) from ERA University (EU), Lucknow.
  • Certificate in Emergency Medical Technician (EMT) Basic with ‘B’ grade from PMKVY in 2017.
  • Master of Science in Biochemistry (2014) from CSJM University Kanpur.
  • Bachelor of Science (Zoology, Botany, Chemistry) (2011) from University of Lucknow, Lucknow.

📚 Scholastic Qualification

  • Higher Secondary (XII) with U.P. Board in 2007.
  • High School (X) with U.P. Board in 2005.

🏷 Positions

  • Research Assistant: Jan 2015 to July 2015, Department of Pathology, ERA’s Lucknow Medical College & Hospital, Lucknow.
  • DBT Junior Research Fellow: July 2015 to March 2019, Department of Obstetrics & Gynecology, ERA’s Lucknow Medical College & Hospital, Lucknow.
  • Research Analyst: March 2019 to present, Research Metabolic Unit, ERA’s Lucknow Medical College & Hospital, Lucknow.

🧑‍🔬 Project/ Research Work Experience

Major Projects

  • Inflammatory Pathway Gene Polymorphisms and Cervical Cancer Progression
    • Title: Co-relative significance of inflammatory pathway gene polymorphisms – Interleukin -1-RN, 1β (-511C/T and +3953C/T) , 6 (174A/G) and 10 (-1082 A/G and -819C/T), with interleukin serum levels and intensity of angiogenesis in the progression of HPV positive cervical cancer
    • Duration: July 2015 to March 2019
    • Location: Department of Obstetrics & Gynecology, ERA’s Lucknow Medical College & Hospital, Lucknow.
  • CYP 46A1 Gene Polymorphism in Cataract
    • Duration: September to December 2013
    • Location: Department of Biotechnology, Central Research Laboratory, ERA’s Lucknow Medical College & Hospital, Lucknow.

📚 Teaching Experience

  • One year teaching experience in Biology with R.P.S. Inter College.
  • Two years of home tuition in Biology.

 

🔬 Biological Cognizance

Biochemistry:

  • Qualitative and Quantitative Analysis of Biomolecules
  • Paper Chromatography, Thin Layer Chromatography, Electrophoretic Techniques

Molecular Biology:

  • DNA and RNA Isolation, Enzyme Kinetics, PCR, RT-PCR, RFLP

Microbiology:

  • Sterilization Techniques, Antibiotic Sensitivity Tests, Microbial Staining, Environmental Monitoring

Immunological Techniques:

  • ELISA, Radioimmunoassay (RIA), Blood Group Identification, Serological Tests

🏆 Awards/ Honors

  • International Best Poster Presentation Award at the 11th International Science Congress (2023) at Pacific University, Udaipur, Rajasthan, India.

Publications Top Notes 📝

  • The application of UDL and SoTL to health care education
    • Year: 2024
    • Authors: Ciolfi, M.; Howard, L.
    • Source: New Directions for Teaching and Learning

 

  • Perceptions of Ontario chiropractors on business education in chiropractic schools
    • Year: 2021
    • Authors: Ciolfi, M.A.; Azad, A.; Al-Azdee, M.; Nguyen, A.; Panah, B.A.
    • Source: Journal of Chiropractic Education

 

  • Gender diversity in chiropractic leadership: a cross-sectional study
    • Year: 2021
    • Authors: Azad, A.; Maiers, M.; Stuber, K.; Ciolfi, M.
    • Source: Journal of the Canadian Chiropractic Association

 

  • Response of Chiropractic Organizations to the COVID-19 Pandemic: A Descriptive Report
    • Year: 2020
    • Authors: Johnson, C.D.; Little, C.S.; Sterling, T.A.; Boghosian, K.; Ciolfi, M.A.
    • Source: Journal of Manipulative and Physiological Therapeutics

 

  • The relationship between chiropractor required and current level of business knowledge
    • Year: 2017
    • Authors: Ciolfi, M.A.; Kasen, P.A.
    • Source: Chiropractic and Manual Therapies

 

Pushpendra D Pratap | Cervical Cancer | Best Researcher Award

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