Dr. Pushpendra D Pratap |Cervical Cancer| Best Researcher Award

🔬 Current Research

I am currently working on:

• Single nucleotide gene polymorphisms (SNPs) of interleukins (ILs) and their association with cervical cancer susceptibility.
• Evaluation of interleukins and other markers’ levels through ELISA.
• Human papillomavirus (HPV) analysis through RT-PCR, a recognized causative agent for cervical cancer and other carcinomas.
• Diagnostic tests for HLA-B-27 and MDTB through RT-PCR.
• COVID-19 testing through RT-PCR.

Additionally, I have experience in the molecular biology of various cancers and other diseases, focusing on signaling pathways, angiogenesis, cell proliferation, apoptosis, autophagy, inflammation, oxidative stress, and targeted therapeutic interventions.

🧪 Expertise

• Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)
• Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
• Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
• KingFisher Duo Prime Extraction System & QS 5Dx qPCR Machine and Analysis Software

🎓 Professional Qualification

• Ph.D. in Biochemistry (2024) from ERA University (EU), Lucknow.
• Certificate in Emergency Medical Technician (EMT) Basic with ‘B’ grade from PMKVY in 2017.
• Master of Science in Biochemistry (2014) from CSJM University Kanpur.
• Bachelor of Science (Zoology, Botany, Chemistry) (2011) from University of Lucknow, Lucknow.

📚 Scholastic Qualification

• Higher Secondary (XII) with U.P. Board in 2007.
• High School (X) with U.P. Board in 2005.

🏷 Positions

• Research Assistant: Jan 2015 to July 2015, Department of Pathology, ERA’s Lucknow Medical College & Hospital, Lucknow.
• DBT Junior Research Fellow: July 2015 to March 2019, Department of Obstetrics & Gynecology, ERA’s Lucknow Medical College & Hospital, Lucknow.
• Research Analyst: March 2019 to present, Research Metabolic Unit, ERA’s Lucknow Medical College & Hospital, Lucknow.

🧑‍🔬 Project/ Research Work Experience

Major Projects

• Inflammatory Pathway Gene Polymorphisms and Cervical Cancer Progression
  • Title: Co-relative significance of inflammatory pathway gene polymorphisms – Interleukin -1-RN, 1β (-511C/T and +3953C/T) , 6 (174A/G) and 10 (-1082 A/G and -819C/T), with interleukin serum levels and intensity of angiogenesis in the progression of HPV positive cervical cancer
  • Duration: July 2015 to March 2019
  • Location: Department of Obstetrics & Gynecology, ERA’s Lucknow Medical College & Hospital, Lucknow.
• CYP 46A1 Gene Polymorphism in Cataract
  • Duration: September to December 2013
  • Location: Department of Biotechnology, Central Research Laboratory, ERA’s Lucknow Medical College & Hospital, Lucknow.

📚 Teaching Experience

• One year teaching experience in Biology with R.P.S. Inter College.
• Two years of home tuition in Biology.

🔬 Biological Cognizance

Biochemistry:

• Qualitative and Quantitative Analysis of Biomolecules
• Paper Chromatography, Thin Layer Chromatography, Electrophoretic Techniques

Molecular Biology:

• DNA and RNA Isolation, Enzyme Kinetics, PCR, RT-PCR, RFLP

Microbiology:

• Sterilization Techniques, Antibiotic Sensitivity Tests, Microbial Staining, Environmental Monitoring

Immunological Techniques:

• ELISA, Radioimmunoassay (RIA), Blood Group Identification, Serological Tests

🏆 Awards/ Honors

• International Best Poster Presentation Award at the 11th International Science Congress (2023) at Pacific University, Udaipur, Rajasthan, India.