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Mr. Daniele Florio | Pharmaceutical science | Young Scientist Award

Daniele Florio is a dedicated researcher in pharmaceutical sciences, currently pursuing a Ph.D. at the University of Naples Federico II. His doctoral work focuses on integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia, scheduled for completion in October 2024. Daniele brings extensive experience from his Marie Sklodowska-Curie secondment and voluntary internship, specializing in peptide synthesis, mass spectrometry, and spectroscopic techniques. With a strong publication record and active participation in international conferences, he contributes significantly to the fields of amyloid aggregation, chemical catalysis, and drug development, showcasing advanced skills in scientific research and analysis.

Profile

Scopus

👨‍🏫Professional Experience

Tutoring Activities (Grant Type B) 2023-2024, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Provided supplementary teaching for Chemistry and Inorganic Chemistry courses for Pharmacy and CTF degree students. Ph.D. Candidate in Pharmaceutical Sciences (XXXVII Cycle) 2021 – Present, Naples, Italy, Department of Pharmacy, University of Naples Federico II, PhD Project: Integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia., Scheduled PhD end: October 31, 2024. Secondee under Marie Skłodowska-Curie Research and Innovation Staff Exchange Action June 2022 – March 2023, Dublin, Ireland, Keladapharmachem, Participated in the GreenX4Drug (EU funded H2020 RISE project), focusing on developing novel asymmetric chemical catalysts for manufacturing high-value APIs and advanced intermediates.. Voluntary Internship Post-Degree 2018 – 2019, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Designed, synthesized, and characterized peptides, peptidomimetics, and small molecules using solid-phase and solution methodologies. Utilized mass spectrometry (ESI) for identifying synthetic compounds and applied analytical and preparative methodologies (HPLC) for their purification and characterization. Conducted structural and functional characterization of biomolecules through spectroscopic techniques (CD, Fluorescence, SPR, UV-Vis). Supervisor: Prof. Daniela Marasco

🎓Education and Training

Biologist Board Examination February 7, 2022, University of Naples Federico II, Naples, Italy Master’s Degree in Biological Science (Molecular Diagnostics Field) July 12, 2021, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Protein fragments of Nucleophosmin 1 as models of amyloids”, Supervisor: Prof. Antonello Merlino, Co-Supervisor: Prof. Daniela Marasco,k Grade: 110/110. Bachelor’s Degree in Biological Science (Molecular Diagnostics Field) March 15, 2018, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Amyloid-like aggregation of Nucleophosmin 1 regions related to Acute Myeloid Leukemia: spectroscopic studies” Supervisor: Prof. Daniela Marasco Grade: 106/110

📖Certifications

English TELSA C1, Grade B Language Certification ESAT, Malta Further & Higher Education Authority

Skills and Competencies

Organizational Skills

  • Efficient under pressure.
  • Adaptive to changes and new situations.
  • Strong independent work organization and goal achievement.

Job-related Skills

  • Solid-phase peptide synthesis and combinatorial peptide libraries using Fmoc/Boc chemistry.
  • Experience with various peptide synthesizers (BIOTAGE, Liberty Blue 2.0).
  • Proficient in analysis and purification using ESI mass spectrometry, HPLC, and flash purification systems.
  • Skilled in spectroscopic techniques (CD, Fluorescence, DLS, SPR, UV-Vis, FTIR, NMR).

Digital Skills

  • Proficient in Microsoft Office Suite.
  • Experienced with operating systems: MS-Windows, MacOS, LINUX.
  • Proficient in scientific data analysis software (GraphPad Prism).
  • Knowledgeable in databases (PubMed) and bibliographic management software (EndNote, Mendeley).
  • Proficient with video conferencing software (Teams, Skype, Zoom, Google Meet).
  • Excellent knowledge of major browsers (Edge, Chrome, Firefox, Safari, Opera).

🌍Projects

National Project Participation

  • BANDO-PRIN-2022. Research Project: Design, synthesis, and investigation of novel G-quadruplex aptamers against STAT3 and the interleukin-6 receptor (G4-APTASTAT).
  • Project Coordinator and Head of Research Unit: Prof. GALEONE Aldo

Bibliometric Parameters

  • H-index from Scopus: 11
  • Number of Publications: 24
  • Citations: 388

 

Publications Top Notes 📝

  • The effects of histidine substitution of aromatic residues on the amyloidogenic properties of the fragment 264–277 of nucleophosmin 1
    • Authors: D. Florio, P. Luciano, C. Di Natale, D. Marasco
    • Journal: Bioorganic Chemistry
    • Volume: 147
    • Year: 2024
    • Pages: 107404

 

  • Could Targeting NPM1c+ Misfolding Be a Promising Strategy for Combating Acute Myeloid Leukemia?
    • Authors: D. Florio, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 25
    • Issue: 2
    • Year: 2024
    • Pages: 811

 

  • Modulation of hydrogel networks by metal ions
    • Authors: S. La Manna, D. Florio, C. Di Natale, D. Marasco
    • Journal: Journal of Peptide Science
    • Volume: 29
    • Issue: 8
    • Year: 2023
    • Article ID: e3474

 

  • Ruthenium complexes bearing glucosyl ligands are able to inhibit the amyloid aggregation of short histidine-peptides
    • Authors: D. Florio, S. La Manna, A. Annunziata, M. Monti, D. Marasco
    • Journal: Dalton Transactions
    • Volume: 52
    • Issue: 25
    • Year: 2023
    • Pages: 8549–8557

 

  • Insights into Network of Hot Spots of Aggregation in Nucleophosmin 1
    • Authors: D. Florio, S. La Manna, C. Di Natale, A.M. Malfitano, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 23
    • Issue: 23
    • Year: 2022
    • Pages: 14704

 

  • Hydrogelation tunability of bioinspired short peptides
    • Authors: S. La Manna, D. Florio, V. Panzetta, C. Di Natale, D. Marasco
    • Journal: Soft Matter
    • Volume: 8
    • Year: 2022
    • Pages: 8

 

Daniele Florio | Pharmaceutical science | Young Scientist Award

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