Yasaman Taslimi | diagnosis | Best Researcher Award

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Dr. Yasaman Taslimi | diagnosis| Autonomous systems | Best Researcher Award

Dr, Pasteur institute of Iran, Iran

šŸ‘©ā€šŸ”¬Ā Dedicated researcher with a Ph.D. in Biology from Pasteur Institute of Iran, specializing in molecular genetics and parasitology. šŸ§¬ Skilled in molecular diagnostics and protein analysis for Leishmaniasis. Currently serving as a Researcher in Immunotherapy and Leishmania Vaccine Research at Pasteur Institute, with extensive teaching and visiting researcher experience globally. Ā Recognized for academic excellence with awards including first rank in Ph.D. studies. Passionate about advancing immunotherapy and infectious disease research, with notable presentations at international congresses and a diverse range of specialized courses attended.šŸŒŸ

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Scholar

 

Education šŸ“š

B.Sc. in Nutrition Azad University, Tehran, Iran 2000, M.Sc. in Biochemistry Azad University, Damghan, Iran 2015, PhD in Biology Pasteur Institute of Iran 2023

Position šŸ’¼

  • Researcher in Immunotherapy and Leishmania Vaccine Research Department
    Pasteur Institute of Iran
    2000-Present

Area of Expertise šŸ§¬

  • Molecular genetics, molecular parasitology, gene function and expression.
  • Molecular diagnosis of Leishmaniasis.
  • Arginase assay.
  • Protein analysis techniques such as Western Blot, Elisa.
  • PEA assay.
  • LAMP assay.

Courses šŸŽ“

Molecular DATA for infectious disease, ITM, Antwerp, Belgium, 2018International Workshop of Leishmania and Leishmaniasis, Pasteur Iran, 2016Molecular Biology of Leishmaniasis, ICGEB, Trieste, Italy, 2016.Targeting and Expression of Gene in Cells, International Congress of Immunology, Tehran, 2014How to write an International Article, Elsevier workshop, Tehran, 2014Application of Bioinformatics in Biotechnology, Pasteur Iran, 2013Flow Cytometry workshop, Pasteur Iran, 2012Quality Control Management, Pasteur Iran, 2012Calibration methods and standards, Pasteur Iran, 2011Life skills courses (Depression, Anger management, Time management, …), Pasteur, 2010Advanced Molecular Genetic course, Pasteur Iran, 2006ICDL courses, 2006

Teaching šŸ“

High throughput technologies in translational immunology, Pasteur, Iran, 2022Western Blot, Practical, School of Pharmacy, Shahid Beheshti University, Tehran, Iran, 2016Immunology of infectious disease, Practical, Pasteur Iran, 2006

Visiting Researcher šŸŒ

Gothenburg University (Leishild-MATI RISE project), Department of Microbiology and Immunology at Institute of Biomedicine, 2019/02/26-2019/05/14Pasteur Institute of Paris (Leishild-MATI RISE project), Department of Molecular Parasitology and Signaling, 2023/12/17-2023/02/17

Congress Presentations šŸ—£ļø

International Congress of Dermatology, Cosmetics and Laser, Iran, Tehran, 2022.International congress of immunology, Iran, Ahvaz, 2021.The 17th International Congress of Immunology, Beijing, China, 2019.Molecular Biology of Leishmania course, Trieste, Italy, 2016.Annual Symposium of the Institut Pasteur International Network, Paris, 2015.6th Worldleish international congress, Toledo, Spain, 2017.

Awards šŸ†

The Second National Conference on New Cellular and Molecular, Iran, 2015.13th International Congress of Immunology and Allergy of Iran, Tabriz, 2015.First ranked Ph.D. student in Pasteur Institute of Iran, 2018.

Publications Top Notes šŸ“

  1. Title: Immunization with the hybrid protein vaccine, consisting of Leishmania major cysteine proteinases Type I (CPB) and Type II (CPA), partially protects against leishmaniasis
    Authors: A Zadeh-Vakili, T Taheri, Y Taslimi, F Doustdari, AH Salmanian, S Rafati
    Journal: Vaccine
    Volume: 22
    Issue: 15-16
    Pages: 1930-1940
    Year: 2004
    Citations: 81

 

  1. Title: Enhanced Protective Efficacy of Nonpathogenic Recombinant Leishmania tarentolae Expressing Cysteine Proteinases Combined with a Sand Fly Salivary Antigen
    Authors: F Zahedifard, E Gholami, T Taheri, Y Taslimi, F Doustdari, N Seyed, …
    Journal: PLoS neglected tropical diseases
    Volume: 8
    Issue: 3
    Pages: e2751
    Year: 2014
    Citations: 79

 

  1. Title: Development of Novel Prime-Boost Strategies Based on a Tri-Gene Fusion Recombinant L. tarentolae Vaccine against Experimental Murine Visceral Leishmaniasis
    Authors: N Saljoughian, T Taheri, F Zahedifard, Y Taslimi, F Doustdari, …
    Journal: PLoS neglected tropical diseases
    Volume: 7
    Issue: 4
    Pages: e2174
    Year: 2013
    Citations: 75

 

  1. Title: Leishmania major heat shock protein 70 (HSP70) is not protective in murine models of cutaneous leishmaniasis and stimulates strong humoral responses in cutaneous and visceral ā€¦
    Authors: S Rafati, E Gholami, N Hassani, F Ghaemimanesh, Y Taslimi, T Taheri, …
    Journal: Vaccine
    Volume: 25
    Issue: 21
    Pages: 4159-4169
    Year: 2007
    Citations: 71

 

  1. Title: Cysteine proteinase type III is protective against Leishmania infantum infection in BALB/c mice and highly antigenic in visceral leishmaniasis individuals
    Authors: N Khoshgoo, F Zahedifard, H Azizi, Y Taslimi, MJ Alonso, S Rafati
    Journal: Vaccine
    Volume: 26
    Issue: 46
    Pages: 5822-5829
    Year: 2008
    Citations: 61

 

 

Somayeh Hashemi Sheikhshabani | Human Genetics | Best Researcher Award

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Dr. Somayeh Hashemi Sheikhshabani | Human Genetics | Best Researcher Award

šŸ‘Øā€šŸ«Profile Summary

I am a highly skilled researcher specializing in Medical Genetics with a focus on Cancer Biology, Cancer Drug Resistance, Noncoding RNAs, Computational Biology, and Genetics of Rare Diseases. With a Ph.D. from Shahid Beheshti University of Medical Sciences, Tehran, Iran, and a strong background in Cellular and Molecular Biology, I’ve conducted extensive research on genetic analysis of male infertility, identification of genes involved in various cancers, and prediction of regulatory networks using bioinformatics tools. Proficient in a wide range of experimental techniques and software applications, I am dedicated to advancing knowledge in genetics and contributing to the development of innovative therapies.

 

šŸŒ Professional Profiles

Education šŸ“š

PhD in Medical Genetics Shahid Beheshti University of Medical Sciences, Tehran, Iran September 23, 2019 ā€“ October 7, 2023 PhD Thesis: Genetic investigation of non-obstructive azoospermia families with cytogenetic and molecular genetic tests, and determining the relationship between genetic and clinical findings of patients.M.Sc. in Cellular and Molecular Biology National Institute of Genetic Engineering and Biotechnology, Tehran, Iran September 23, 2010 ā€“ October 27, 2012 Master Thesis: Alkaloid extract of Peganum harmala L. seed on the expression of Bax and Bcl-2 genes involved in mitochondrial apoptosis pathway in cancer cells.B.Sc. in Molecular Genetics Shahrekord University, Shahrekord, Iran September 23, 2005 ā€“ October 7, 2009

Research Interests šŸ”¬

  • Cancer Biology
  • Cancer Drug Resistance
  • Noncoding RNAs
  • Computational Biology
  • Genetics of Rare Diseases

Research Projects šŸ§¬

Genetic analysis of male infertility in a family using Whole Exome Sequencing. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)In silico prediction of non-coding RNA-gene regulatory network in primary gonad failure defects. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)In silico identification of functional single nucleotide polymorphisms of azoospermia genes. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)In silico prediction of coagulation system functional polymorphisms. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)Identification of genes involved in Imatinib resistance in chronic myeloid leukemia (CML). (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)Genetic analysis of families with non-obstructive azoospermia by Whole Exome Sequencing. (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2024)Genetic investigation of infertility in an Iranian family with infertility in both sexes. (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2023)Prediction of tissue-specific inflammatory LncRNAs in COVID-19 patients. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2021-2024)Identification of inflammatory miRNAs associated with COVID-19 and determination of their potential target genes using bioinformatics tools. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2021-2023)Identification of genes involved in the development of chemotherapy resistance in lung cancer. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2021-2024)Identification of common polymorphisms in the host antioxidant pathway against viruses and prediction of their effects. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2021-2024)Identification of genes involved in the development of chemotherapy resistance in ovarian cancer. (Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2021-2023)Investigation of the effect of oleuropein on miR-125b and miR-34a expression in ovarian cancer cell lines. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2019-2020)Investigation of the effect of oleuropein on miR-181b and miR-21 expression in breast cancer cell line. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2019-2020)Investigation of the effect of oleuropein on the expression of genes involved in apoptosis in cisplatin-resistant ovarian cell line. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2019-2020)Study of the effect of nanoparticles containing altretiamine and arstin on ferroptosis in ovarian cancer cell line. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2016-2020)Study of the effect of nanoparticles containing altretamine on ferroptosis-apoptosis in ovarian cancer cell line. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2018-2019)Genetic etiology determination of non-syndromic hearing loss families using Whole Exome Sequencing. (Cellular and Molecular Research Center, Shahrekord University of Medical Sciences; 2018-2019)

Skills šŸ› ļø

A. Experimental Techniques:Human cell cultureMTT assay (Drugs, Natural products, Nanoparticles)DNA and RNA extractionApoptosis assayROS assayElectrophoresisQuantitative PCRConventional PCRSonicationNanodropKaryotypingSanger sequencingWhole exome sequencingB. Software Ability:Various microRNA databases (TargetScan, miRBase, miRWalk, etc.)Various lncRNA databases (starBase, LncBase, etc.)Pathway databases (KEGG, WikiPathways, Reactome)Variant prediction tools (SIFT, Mutation Taster, Polyphen, CADD, etc.)Genome browsers (NCBI, Ensembl, UCSC)Primer design software and tools (AlleleID, Primer3, Oligo 7, etc.)Sequence analysis software (QIAGEN CLC Genomics Workbench, Mutation Surveyor, FinchTV, etc.)STRINGDBCytoscapeGene RunnerGenome expression databases (GEO, GTEx, etc.)

Workshops šŸ“š

 

  • Various workshops covering topics such as predicting LncRNAs, the role of biological systems in cancer, electronic resource investigation, Next Generation sequencing, molecular techniques, article writing, PCR, and bioinformatics.

šŸ“šTop Noted Publication

  1. Title: The effect of oleuropein on apoptotic pathway regulators in breast cancer cells
    • Journal: European Journal of Pharmacology
    • Year: 2020
    • Volume: 886
    • Pages: 173509
    • Citations: 43

 

  1. Title: Peganum harmala L.ā€™s anti-growth effect on a breast cancer cell line
    • Journal: Biotechnology reports
    • Year: 2015
    • Volume: 8
    • Pages: 138-143
    • Citations: 39

 

  1. Title: Oleuropein reduces cisplatin resistance in ovarian cancer by targeting apoptotic pathway regulators
    • Journal: Life Sciences
    • Year: 2021
    • Volume: 278
    • Pages: 119525
    • Citations: 26

 

  1. Title: Growth inhibitory impact of Peganum harmala L. on two breast cancer cell lines
    • Journal: Iranian journal of biotechnology
    • Year: 2014
    • Volume: 12
    • Issue: 1
    • Pages: 8-14
    • Citations: 23

 

  1. Title: The impact of oleuropein on miRNAs regulating cell death signaling pathway in human cervical cancer cells
    • Journal: Biotechnology and Applied Biochemistry
    • Year: 2024
    • Volume: 71
    • Issue: 1
    • Pages: 61-71
    • Citations: 1

 

  1. Title: In Silico Prediction of Functional SNPs Interrupting Antioxidant Defense Genes in Relation to COVID-19 Progression
    • Journal: Biochemical Genetics
    • Year: 2024
    • Pages: 1-27

 

  1. Title: Meta-analysis of microarray data to determine gene indicators involved in the cisplatin resistance in ovarian cancer
    • Journal: Cancer Reports
    • Year: 2023
    • Volume: 6
    • Issue: 12
    • Pages: e1884

Flavia | Molecular Biology | Young Scientist Award

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Dr. Flavia| Molecular Biology | Young Scientist Award

šŸ‘Øā€šŸ«Profile Summary

Flavia Scoyni is a highly accomplished researcher in molecular medicine, specializing in non-coding RNAs and their role in neurodegenerative diseases like Alzheimer’s and ischemic stroke. With a Ph.D. from the University of Eastern Finland under the Marie Skłodowska-Curie Programme, Flavia has extensive experience in experimental techniques such as scRNA-seq, FACS, and animal modeling. Their work has been published in prestigious journals and recognized with numerous grants and fellowships. Flavia’s expertise in molecular biology, cell culture, and animal work, coupled with a strong track record of securing research funding, positions them as a valuable asset in the field of translational medicine.

šŸŒ Professional Profiles

šŸŽ“Education:

Ph.D. in Molecular Medicine University of Eastern Finland, Kuopio, Finland Duration: Dec 2017 – Feb 2024 Marie Skłodowska-Curie Programme in Genetics and Mechanisms in Translational Medicine (GENOMMED) Advisor: Prof. Tarja Malm Dissertation: “Unlocking the potential of non-coding RNAs in regulating neurodegeneration – a glimpse into Alzheimerā€™s disease and ischemic stroke pathophysiology.” Ph.D. secondment Aarhus University, Aarhus, Denmark Duration: Sept 2018 – Feb 201 Advisor: Assoc. Prof. Thomas B. Hansen Funded by Saastamoinen Foundation travel grant FELASA accreditationUniversity of Eastern Finland, Kuopio, Finland May 2017 Federation of Laboratory Animal Science Associations (FELASA) Certificate in laboratory animal science for EU Functions A/D MSc in Molecular Biology “Sapienza” University of Rome, Rome, Italy Duration: Oct 2013 – Dec 2015 Master Degree in Molecular Biology Graduated magna cum laude (110/110 cum laude) Advisor: Prof. Alessandro Fatica Thesis title: “Identification of new transcriptional targets of p30 C/EBPĪ± transcription factor isoform in acute myeloid leukemia” MSc in Genetics University Paris-Diderot VII, Paris, France Duration: Oct 2013 – June 2015 BSc in Biotechnology “Sapienza” University of Rome, Rome, Italy Duration: Oct 2010 – July 2013 Bachelor degree in Biotechnology Graduated magna cum laude (110/110 cum laude) Advisor: Prof. Carlo Presutti Thesis title: “The role of the mir-200 family in the regulation of the expression of AP1 transcriptional factor”

šŸ”¬Research Experience:

Early stage researcher Professor Tarja Malm University of Eastern Finland, A.I. Virtanen Institute for Molecular Sciences Jan 2017 ā€“ Dec 2017 Investigated the role of microRNAs in phagocytic microglia isolated from adult Alzheimerā€™s mice. Developed techniques including ex-vivo FACS, low input RNA extraction, cloning, qPCR, and protocol for transfection for hard-to-transfect cell lines. Conducted animal procedures to harvest brain samples or acutely isolate adult microglia. Erasmus traineeship Biotech Research & Innovation Centre, Copenhagen, Denmark Oct 2016 – June 2016 Developed a transgenic stable cell line for long non-coding RNAs. Applied techniques such as cloning of long inserts, lentiviral vector production, and stable cell line development. Master thesis Professor Alessandro Fatica “Sapienza” University of Rome, Rome, Italy June 2014 – Dec 2015 Investigated the regulation of the long non-coding RNA UCA1 mediated by C/EBPĪ± transcription factor in the K562 lymphoblast cell line. Applied techniques including ChIP, cloning, RNA extraction, RT-qPCR, WB, transfection, CRISPR-Cas9, and stable cell line development. Master Internship Professor Giuseppe Macino “Sapienza” University of Rome, Rome, Italy Sept 2013 – March 2014 Studied microRNAs inducing epigenetic modifications in mammals.Applied techniques such as ChIP, cloning, mutagenesis, RNA extraction, RT-qPCR, WB, CRISPR-Cas9, and stable cell line development. Bachelor thesis Professor Carlo Presutti “Sapienza” University of Rome, Rome, Italy Oct 2012 – July 2013 Investigated the role of the miR-200a family in regulating c-JUN transcription factor expression. Utilized techniques including cloning, mutagenesis, bacterial transformation, and cell line culture.

šŸ’» Technical Skills:

Molecular biology techniques such as scRNA-seq, RNA-seq, FACS, cloning, RT-qPCR, WB, lentiviral vectors generation. Cell culture techniques including neuronal culture, primary microglial culture, transduction, transfection, and stable cell line development. Animal work including FELASA A/D certification, maintenance, and breeding of transgenic mouse models, animal procedures, and tamoxifen-induced recombination. Statistical analysis, basic Python, and R.

šŸ†Fellowships, Awards, and Honors:

  • Secured total grants of 99.000ā‚¬ during Ph.D., including grants from Pavo Nuermen Foundation, Finnish Cultural Foundation, Kuopio University Foundation, and others.

šŸ‘©ā€šŸ« Teaching Experience:

  • Mentored a Ph.D. student and post-graduate student, and supervised five master’s thesis students during the Ph.D. program.

šŸ“šTop Noted Publication

  1. ciRS-7 and miR-7 regulate ischemia-induced neuronal death via glutamatergic signaling
    • Journal: Cell Reports
    • Year: 2024-03
    • DOI: 10.1016/j.celrep.2024.113862
    • Contributors: Flavia Scoyni; Valeriia Sitnikova; Luca Giudice; Paula Korhonen; Davide M. Trevisan; Ana Hernandez de Sande; Mireia Gomez-Budia; Raisa Giniatullina; Irene F. Ugidos; Hiramani Dhungana et al.

 

  1. Alzheimer’s disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAs
    • Journal: Alzheimer’s & Dementia
    • Year: 2024-02
    • DOI: 10.1002/alz.13502
    • Contributors: Flavia Scoyni; Luca Giudice; Mari-Anna VƤƤnƤnen; Nicholas Downes; Paula Korhonen; Xin Yi Choo; Nelli-Noora VƤlimƤki; Petri MƤkinen; Nea Korvenlaita; Annemieke J Rozemuller et al.

 

  1. ciRS-7 and miR-7 regulate ischemia induced neuronal death via glutamatergic signaling
    • Journal: Preprint
    • Year: 2023-01-24
    • DOI: 10.1101/2023.01.24.525136
    • Contributors: Flavia Scoyni; Valeriia Sitnikova; Luca Giudice; Paula Korhonen; Davide M Trevisan; Ana Hernandez de Sande; Mireia Gomez-Budia; Raisa Giniatullina; Irene F Ugidos; Hiramani Dhungana et al.

 

  1. Intracerebral overexpression of miR-669c is protective in mouse ischemic stroke model by targeting MyD88 and inducing alternative microglial/macrophage activation
    • Journal: Journal of Neuroinflammation
    • Year: 2020
    • DOI: 10.1186/s12974-020-01870-w
    • Contributors: Kolosowska, N.; Gotkiewicz, M.; Dhungana, H.; Giudice, L.; Giugno, R.; Box, D.; Huuskonen, M.T.; Korhonen, P.; Scoyni, F.; Kanninen, K.M. et al.

 

  1. PSEN1Ī”E9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia
    • Journal: Stem Cell Reports
    • Year: 2019
    • DOI: 10.1016/j.stemcr.2019.08.004
    • Contributors: Konttinen, H.; Cabral-da-Silva, M.E.C.; Ohtonen, S.; Wojciechowski, S.; Shakirzyanova, A.; Caligola, S.; Giugno, R.; Ishchenko, Y.; HernĆ”ndez, D.; Fazaludeen, M.F. et al.