Daniele Florio | Pharmaceutical science | Young Scientist Award

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Mr. Daniele Florio | Pharmaceutical science | Young Scientist Award

Daniele Florio is a dedicated researcher in pharmaceutical sciences, currently pursuing a Ph.D. at the University of Naples Federico II. His doctoral work focuses on integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia, scheduled for completion in October 2024. Daniele brings extensive experience from his Marie Sklodowska-Curie secondment and voluntary internship, specializing in peptide synthesis, mass spectrometry, and spectroscopic techniques. With a strong publication record and active participation in international conferences, he contributes significantly to the fields of amyloid aggregation, chemical catalysis, and drug development, showcasing advanced skills in scientific research and analysis.

Profile

Scopus

👨‍🏫Professional Experience

Tutoring Activities (Grant Type B) 2023-2024, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Provided supplementary teaching for Chemistry and Inorganic Chemistry courses for Pharmacy and CTF degree students. Ph.D. Candidate in Pharmaceutical Sciences (XXXVII Cycle) 2021 – Present, Naples, Italy, Department of Pharmacy, University of Naples Federico II, PhD Project: Integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia., Scheduled PhD end: October 31, 2024. Secondee under Marie Skłodowska-Curie Research and Innovation Staff Exchange Action June 2022 – March 2023, Dublin, Ireland, Keladapharmachem, Participated in the GreenX4Drug (EU funded H2020 RISE project), focusing on developing novel asymmetric chemical catalysts for manufacturing high-value APIs and advanced intermediates.. Voluntary Internship Post-Degree 2018 – 2019, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Designed, synthesized, and characterized peptides, peptidomimetics, and small molecules using solid-phase and solution methodologies. Utilized mass spectrometry (ESI) for identifying synthetic compounds and applied analytical and preparative methodologies (HPLC) for their purification and characterization. Conducted structural and functional characterization of biomolecules through spectroscopic techniques (CD, Fluorescence, SPR, UV-Vis). Supervisor: Prof. Daniela Marasco

🎓Education and Training

Biologist Board Examination February 7, 2022, University of Naples Federico II, Naples, Italy Master’s Degree in Biological Science (Molecular Diagnostics Field) July 12, 2021, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Protein fragments of Nucleophosmin 1 as models of amyloids”, Supervisor: Prof. Antonello Merlino, Co-Supervisor: Prof. Daniela Marasco,k Grade: 110/110. Bachelor’s Degree in Biological Science (Molecular Diagnostics Field) March 15, 2018, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Amyloid-like aggregation of Nucleophosmin 1 regions related to Acute Myeloid Leukemia: spectroscopic studies” Supervisor: Prof. Daniela Marasco Grade: 106/110

📖Certifications

English TELSA C1, Grade B Language Certification ESAT, Malta Further & Higher Education Authority

Skills and Competencies

Organizational Skills

  • Efficient under pressure.
  • Adaptive to changes and new situations.
  • Strong independent work organization and goal achievement.

Job-related Skills

  • Solid-phase peptide synthesis and combinatorial peptide libraries using Fmoc/Boc chemistry.
  • Experience with various peptide synthesizers (BIOTAGE, Liberty Blue 2.0).
  • Proficient in analysis and purification using ESI mass spectrometry, HPLC, and flash purification systems.
  • Skilled in spectroscopic techniques (CD, Fluorescence, DLS, SPR, UV-Vis, FTIR, NMR).

Digital Skills

  • Proficient in Microsoft Office Suite.
  • Experienced with operating systems: MS-Windows, MacOS, LINUX.
  • Proficient in scientific data analysis software (GraphPad Prism).
  • Knowledgeable in databases (PubMed) and bibliographic management software (EndNote, Mendeley).
  • Proficient with video conferencing software (Teams, Skype, Zoom, Google Meet).
  • Excellent knowledge of major browsers (Edge, Chrome, Firefox, Safari, Opera).

🌍Projects

National Project Participation

  • BANDO-PRIN-2022. Research Project: Design, synthesis, and investigation of novel G-quadruplex aptamers against STAT3 and the interleukin-6 receptor (G4-APTASTAT).
  • Project Coordinator and Head of Research Unit: Prof. GALEONE Aldo

Bibliometric Parameters

  • H-index from Scopus: 11
  • Number of Publications: 24
  • Citations: 388

 

Publications Top Notes 📝

  • The effects of histidine substitution of aromatic residues on the amyloidogenic properties of the fragment 264–277 of nucleophosmin 1
    • Authors: D. Florio, P. Luciano, C. Di Natale, D. Marasco
    • Journal: Bioorganic Chemistry
    • Volume: 147
    • Year: 2024
    • Pages: 107404

 

  • Could Targeting NPM1c+ Misfolding Be a Promising Strategy for Combating Acute Myeloid Leukemia?
    • Authors: D. Florio, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 25
    • Issue: 2
    • Year: 2024
    • Pages: 811

 

  • Modulation of hydrogel networks by metal ions
    • Authors: S. La Manna, D. Florio, C. Di Natale, D. Marasco
    • Journal: Journal of Peptide Science
    • Volume: 29
    • Issue: 8
    • Year: 2023
    • Article ID: e3474

 

  • Ruthenium complexes bearing glucosyl ligands are able to inhibit the amyloid aggregation of short histidine-peptides
    • Authors: D. Florio, S. La Manna, A. Annunziata, M. Monti, D. Marasco
    • Journal: Dalton Transactions
    • Volume: 52
    • Issue: 25
    • Year: 2023
    • Pages: 8549–8557

 

  • Insights into Network of Hot Spots of Aggregation in Nucleophosmin 1
    • Authors: D. Florio, S. La Manna, C. Di Natale, A.M. Malfitano, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 23
    • Issue: 23
    • Year: 2022
    • Pages: 14704

 

  • Hydrogelation tunability of bioinspired short peptides
    • Authors: S. La Manna, D. Florio, V. Panzetta, C. Di Natale, D. Marasco
    • Journal: Soft Matter
    • Volume: 8
    • Year: 2022
    • Pages: 8

 

Virginia M. Concato-Lopes | drug-discovery | Best Researcher Award

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Dr. Virginia M. Concato-Lopes | toxicology Award |Best Researcher Award

👨‍🏫Profile Summary

With a Ph.D. and Master’s in Experimental Pathology from Universidade Estadual de Londrina, Brazil, and ongoing postdoctoral research at Cedars-Sinai Medical Center, I specialize in the biogenesis of large oncosomes. My academic journey includes a Bachelor’s in Biomedicine from Universidade do Norte do Paraná. I’ve provided academic advisory for research projects investigating the antiproliferative properties of oregano essential oil in cancer cells. Passionate about advancing biomedical knowledge, I contribute to cutting-edge research and mentorship initiatives. For further information, please refer to my published papers or contact the respective academic institutions directly.

🌐 Professional Profiles

🎓 Education

Postdoctoral Fellow in Biogenesis of Large Oncosomes Cedars-Sinai Medical Center, Los Angeles, California, United States (2023 – Current), Ph.D. in Experimental Pathology Universidade Estadual de Londrina, Londrina, Paraná, Brazil (2019 – 2023), Master in Experimental Pathology
Universidade Estadual de Londrina, Londrina, Paraná, Brazil (2017 – 2019) Bachelor of Biomedicine Universidade do Norte do Paraná, Paraná, Brazil (2013 – 2016).

👨‍🏫Academic Advisory

Milena Cremer de Souza: Evaluating the antiproliferative capacity of Oregano Essential Oil (Origanum vulgare) on PC-3 prostate cancer cells. (2022) Degree: Ciências Biológicas (Biological Sciences) Institution: Universidade Estadual do Norte do Paraná Funding: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Maria Beatriz Madureira: Exploring the antiproliferative activity of oregano oil in liver carcinoma tumor lines. (2021) Degree: Biomedicine Institution: Philadelphia University Center  For further details, please refer to the respective academic papers or contact the institutions directly.

Published article (first author publication)

  1. Concato-Lopes, V. M., Gonçalves-Lens, M. D., Tomiotto-Pellissier, F., Detoni, M. B., Cruz, E. M. S., Bortoleti, B. T. D. S., Carloto, A. C. M., Rodrigues, A. C. J., Silva, T. F., Siqueira, E. D. S., De Matos, R. L. N., Alves Cardoso, I. L., Conchon-Costa, I., Lazarin-Bidoia, D., Arakawa, N. S., Dekker, R. F., Mantovani, M. S., & Pavanelli, W. R. Trilobolide-6-O-isobutyrate from Sphagneticola trilobata acts by inducing oxidative stress, metabolic changes and apoptosis-like processes by caspase 3/7 activation of human lung cancer cell lines. Phytomedicine, v. 155536, 2024.
  1. Concato-Lopes, V. M., Silva, T. F., Detoni, M. B., Cruz, E. M. S., Gonçalves, M. D., Da Silva Bortoleti, B. T., Tomiotto-Pellissier, F., Carloto, A. C. M., Madureira, M. B., Rodrigues, A. C. J., Schirmann, J. G., Barbosa-Dekker, A. M., Dekker, R. F., Conchon-Costa, I., Panis, C., Lazarin-Bidóia, D., Miranda-Sapla, M. M., Mantovani, M. S., & Pavanelli, W. R. 3,3′,5,5′-Tetramethoxybiphenyl-4,4’diol triggers oxidative stress, metabolic changes, and apoptosis-like process by reducing the PI3K/AKT/NF-κB pathway in the NCI-H460 lung cancer cell line. Biomedicine & Pharmacotherapy, 170, 115979, 2024.
  1. Concato, Virginia Marcia; Tomiotto-Pellissier, Fernanda; Silva, Taylon Felipe; Gonçalves, Manoela Daiele; Da Silva Bortoleti, Bruna Taciane; Detoni, Mariana Barbosa; Da Silva Siqueira, Elaine; Jacob Rodrigues, Ana Carolina; Schirmann, Jéseka Gabriela; De Melo Barbosa-Dekker, Aneli; Costa, Idessania Nazareth; Conchon-Costa, Ivete; Miranda-Sapla, Milena Menegazzo; Mantovani, Mario Sergio; Pavanelli, Wander Rogério. 3,3-,5,5–tetramethoxybiphenyl-4,4-diol induces cell cycle arrest in G2/M phase and apoptosis in human non-small cell lung cancer A549-cells. Chemico-Biological Interactions, v. 2020, p. 109133, 2020.

 

Published book chapters

  1. Silva, T. F.; Tomiotto-Pellissier, F.; Sanfelice, Raquel Arruda; Gonçalves, Manoela Daiele; Bortoleti, Bruna; Detoni, Mariana Barbosa; Rodrigues, Ana Carolina Jacob; Carloto, Amanda; Concato, Virginia Marcia; Siqueira, Elaine Da Silva; Costa, I. N.; Pavanelli, Wander; Conchon-Costa, Ivete; Miranda-Sapla, M. M. A 21st Century Evil: Immunopathology and New Therapies of COVID-19 In: CORONAVIRUS DISEASE (COVID-19): PATHOPHYSIOLOGY, EPIDEMIOLOGY, CLINICAL MANAGEMENT AND PUBLIC HEALTH RESPONSE.1 ed.Lausanne: Frontiers Media SA, 2023, v.C, p. 164-194.

 

  1. Fagundes, Tatiane Renata; Kawassaki, A. C. B.; Concato, Virginia Marcia; Assolini, João Paulo; Silva, Taylon Felipe; Goncalves, M. D.; Da Silva Siqueira, Elaine; Sahd, C. S.; Inoue, Fabricio Seidy Ribeiro; Silva, T. P.; Lima, D. M.; Ferreira, Mariane Okamoto; Conchon-Costa, Ivete; Pavanelli, W. R.; Panis, Carolina. Impact of Pesticides on Immune-Endocrine Disorders and Its Relationship to Cancer Development In: Handbook of Cancer and Immunology.30, 2023, p. 1-30.

 

  1. Tomiotto-Pellissier, Fernanda; Gonçalves, Manoela Daiele; Silva, Taylon Felipe; Concato, Virgínia Márcia; Bortoleti, Bruna Taciane da Silva; Arakawa, Nilton Syogo; Conchon-Costa, Ivete; Pavanelli, Wander Rogério; and Panis, Carolina. Structure-Activity Relationship (SAR) Studies on Anti-Cancer Bioactive Natural Products. In: Atta-ur-Rahman. Studies in Natural Products Chemistry. Netherlands: Elsevier. Volume 72, 1st edition, 2022. ISBN: 978-0-128-23944-5.

Published book chapters

  1. Silva, T. F.; Tomiotto-Pellissier, F.; Sanfelice, Raquel Arruda; Gonçalves, Manoela Daiele; Bortoleti, Bruna; Detoni, Mariana Barbosa; Rodrigues, Ana Carolina Jacob; Carloto, Amanda; Concato, Virginia Marcia; Siqueira, Elaine Da Silva; Costa, I. N.; Pavanelli, Wander; Conchon-Costa, Ivete; Miranda-Sapla, M. M. A 21st Century Evil: Immunopathology and New Therapies of COVID-19 In: CORONAVIRUS DISEASE (COVID-19): PATHOPHYSIOLOGY, EPIDEMIOLOGY, CLINICAL MANAGEMENT AND PUBLIC HEALTH RESPONSE.1 ed.Lausanne: Frontiers Media SA, 2023, v.C, p. 164-194.

 

  1. Fagundes, Tatiane Renata; Kawassaki, A. C. B.; Concato, Virginia Marcia; Assolini, João Paulo; Silva, Taylon Felipe; Goncalves, M. D.; Da Silva Siqueira, Elaine; Sahd, C. S.; Inoue, Fabricio Seidy Ribeiro; Silva, T. P.; Lima, D. M.; Ferreira, Mariane Okamoto; Conchon-Costa, Ivete; Pavanelli, W. R.; Panis, Carolina. Impact of Pesticides on Immune-Endocrine Disorders and Its Relationship to Cancer Development In: Handbook of Cancer and Immunology.30, 2023, p. 1-30.

 

  1. Tomiotto-Pellissier, Fernanda; Gonçalves, Manoela Daiele; Silva, Taylon Felipe; Concato, Virgínia Márcia; Bortoleti, Bruna Taciane da Silva; Arakawa, Nilton Syogo; Conchon-Costa, Ivete; Pavanelli, Wander Rogério; and Panis, Carolina. Structure-Activity Relationship (SAR) Studies on Anti-Cancer Bioactive Natural Products. In: Atta-ur-Rahman. Studies in Natural Products Chemistry. Netherlands: Elsevier. Volume 72, 1st edition, 2022. ISBN: 978-0-128-23944-5.

 Published Abstracts

 

  1. Rodrigues, A. C. J.; Silva, T. F.; Detoni, Mariana Barbosa; Concato, Virginia Marcia; Tomiotto-Pelissier, Fernanda; Miranda-Sapla, M. M.; Bortoleti, Bruna; Gonçalves, M. D.; Carloto, Amanda; Sanfelice, Raquel Arruda; Cruz, Ellen Mayara Sousa ; Morais, J. M. B.; Bidóia, Danielle Larazin; Costa, I. N.; Pavanelli, W. R.; Conchon-Costa, I. A hiperglicemia modula a capacidade leishmanicida dos macrófagos, com redução de mediadores pró inflamatórios e aumento na atividade enzimática de sod e catalase In: XIII Simpósio Sul de Imunologia, 2022, Blumenal – SC. XIII Simpósio Sul de Imunologia. , 2022.

 

  1. Carloto, Amanda; Silva, T. F.; Detoni, Mariana Barbosa; Concato, Virginia Marcia; Tomiotto-Pelissier, Fernanda; Miranda-Sapla, M. M.; Bortoleti, Bruna; Gonçalves, Manoela Daiele; Rodrigues, Ana Carolina Jacob; Sanfelice, Raquel Arruda; Cruz, Ellen Mayara Sousa; Bidóia, Danielle Larazin; Costa, I. N.; Pavanelli, W. R.; Conchon-Costa, I. Diabetes reduz estresse oxidativo e favorece a infecção em camundongos c57bl/6 por leishmania amazonensis In: XIII Simpósio Sul de Imunologia, 2022, Blumenal – SC. XIII Simpósio Sul de Imunologia. , 2022.

 

  1. Caputo, W. L.; Silva, T. F.; Detoni, Mariana Barbosa; Concato, Virginia Marcia; Tomiotto-Pellissier, F.; Miranda-Sapla, M. M.; Bortoleti, Bruna; Gonçalves, M. D.; Jacob Rodrigues, Ana Carolina; Sanfelice, Raquel Arruda; Cruz, Ellen Mayara Sousa ; Carloto, Amanda; Bidóia, Danielle Larazin; Costa, I. N.; Pavanelli, W. R.; Conchon-Costa, I. Estímulo hiperglicêmico altera a resposta macrofágica desencadeada pela infecção por leishmania amazonensis In: XIII Simpósio Sul de Imunologia, 2022, Blumenal – SC. XIII Simpósio Sul de Imunologia. , 2022.

 

📚Top Noted Publication

 

3,3′,5,5′-Tetramethoxybiphenyl-4,4’diol triggers oxidative stress, metabolic changes, and apoptosis-like process by reducing the PI3K/AKT/NF-κB pathway in the NCI-H460 lung cancer cell line