Mr. Daniele Florio | Pharmaceutical science | Young Scientist Award

Daniele Florio is a dedicated researcher in pharmaceutical sciences, currently pursuing a Ph.D. at the University of Naples Federico II. His doctoral work focuses on integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia, scheduled for completion in October 2024. Daniele brings extensive experience from his Marie Sklodowska-Curie secondment and voluntary internship, specializing in peptide synthesis, mass spectrometry, and spectroscopic techniques. With a strong publication record and active participation in international conferences, he contributes significantly to the fields of amyloid aggregation, chemical catalysis, and drug development, showcasing advanced skills in scientific research and analysis.

Profile

Scopus

👨‍🏫Professional Experience

Tutoring Activities (Grant Type B) 2023-2024, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Provided supplementary teaching for Chemistry and Inorganic Chemistry courses for Pharmacy and CTF degree students. Ph.D. Candidate in Pharmaceutical Sciences (XXXVII Cycle) 2021 – Present, Naples, Italy, Department of Pharmacy, University of Naples Federico II, PhD Project: Integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia., Scheduled PhD end: October 31, 2024. Secondee under Marie Skłodowska-Curie Research and Innovation Staff Exchange Action June 2022 – March 2023, Dublin, Ireland, Keladapharmachem, Participated in the GreenX4Drug (EU funded H2020 RISE project), focusing on developing novel asymmetric chemical catalysts for manufacturing high-value APIs and advanced intermediates.. Voluntary Internship Post-Degree 2018 – 2019, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Designed, synthesized, and characterized peptides, peptidomimetics, and small molecules using solid-phase and solution methodologies. Utilized mass spectrometry (ESI) for identifying synthetic compounds and applied analytical and preparative methodologies (HPLC) for their purification and characterization. Conducted structural and functional characterization of biomolecules through spectroscopic techniques (CD, Fluorescence, SPR, UV-Vis). Supervisor: Prof. Daniela Marasco

🎓Education and Training

Biologist Board Examination February 7, 2022, University of Naples Federico II, Naples, Italy Master’s Degree in Biological Science (Molecular Diagnostics Field) July 12, 2021, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Protein fragments of Nucleophosmin 1 as models of amyloids”, Supervisor: Prof. Antonello Merlino, Co-Supervisor: Prof. Daniela Marasco,k Grade: 110/110. Bachelor’s Degree in Biological Science (Molecular Diagnostics Field) March 15, 2018, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Amyloid-like aggregation of Nucleophosmin 1 regions related to Acute Myeloid Leukemia: spectroscopic studies” Supervisor: Prof. Daniela Marasco Grade: 106/110

📖Certifications

English TELSA C1, Grade B Language Certification ESAT, Malta Further & Higher Education Authority

Skills and Competencies

Organizational Skills

Efficient under pressure.
Adaptive to changes and new situations.
Strong independent work organization and goal achievement.

Job-related Skills

Solid-phase peptide synthesis and combinatorial peptide libraries using Fmoc/Boc chemistry.
Experience with various peptide synthesizers (BIOTAGE, Liberty Blue 2.0).
Proficient in analysis and purification using ESI mass spectrometry, HPLC, and flash purification systems.
Skilled in spectroscopic techniques (CD, Fluorescence, DLS, SPR, UV-Vis, FTIR, NMR).

Digital Skills

Proficient in Microsoft Office Suite.
Experienced with operating systems: MS-Windows, MacOS, LINUX.
Proficient in scientific data analysis software (GraphPad Prism).
Knowledgeable in databases (PubMed) and bibliographic management software (EndNote, Mendeley).
Proficient with video conferencing software (Teams, Skype, Zoom, Google Meet).
Excellent knowledge of major browsers (Edge, Chrome, Firefox, Safari, Opera).

🌍Projects

National Project Participation

BANDO-PRIN-2022. Research Project: Design, synthesis, and investigation of novel G-quadruplex aptamers against STAT3 and the interleukin-6 receptor (G4-APTASTAT).
Project Coordinator and Head of Research Unit: Prof. GALEONE Aldo

Bibliometric Parameters

H-index from Scopus: 11
Number of Publications: 24
Citations: 388

Publications Top Notes 📝

The effects of histidine substitution of aromatic residues on the amyloidogenic properties of the fragment 264–277 of nucleophosmin 1
- Authors: D. Florio, P. Luciano, C. Di Natale, D. Marasco
- Journal: Bioorganic Chemistry
- Volume: 147
- Year: 2024
- Pages: 107404

Could Targeting NPM1c+ Misfolding Be a Promising Strategy for Combating Acute Myeloid Leukemia?
- Authors: D. Florio, D. Marasco
- Journal: International Journal of Molecular Sciences
- Volume: 25
- Issue: 2
- Year: 2024
- Pages: 811

Modulation of hydrogel networks by metal ions
- Authors: S. La Manna, D. Florio, C. Di Natale, D. Marasco
- Journal: Journal of Peptide Science
- Volume: 29
- Issue: 8
- Year: 2023
- Article ID: e3474

Ruthenium complexes bearing glucosyl ligands are able to inhibit the amyloid aggregation of short histidine-peptides
- Authors: D. Florio, S. La Manna, A. Annunziata, M. Monti, D. Marasco
- Journal: Dalton Transactions
- Volume: 52
- Issue: 25
- Year: 2023
- Pages: 8549–8557

Insights into Network of Hot Spots of Aggregation in Nucleophosmin 1
- Authors: D. Florio, S. La Manna, C. Di Natale, A.M. Malfitano, D. Marasco
- Journal: International Journal of Molecular Sciences
- Volume: 23
- Issue: 23
- Year: 2022
- Pages: 14704

Hydrogelation tunability of bioinspired short peptides
- Authors: S. La Manna, D. Florio, V. Panzetta, C. Di Natale, D. Marasco
- Journal: Soft Matter
- Volume: 8
- Year: 2022
- Pages: 8

Pallab Mandal :Leading Researcher in Jadavpur University.

👨‍🏫 Pallab Mandal , an Mr at Jadavpur University. stands as a distinguished academic and researcher in the Management and Accounting. Holding PhD Scholar: Pursuing from Jadavpur University Master of Pharmacy: Jadavpur University (2019), India. 🌟Pallab Mandal is a dedicated professional with extensive experience in bio-analytical method development, scientific/medical writing, and profound expertise in breast cancer research. His journey showcases a robust background in pharmaceuticals, with a strong focus on bioequivalence studies, LC-MS/MS analysis, and pharmacokinetic investigations. He possesses a comprehensive skill set in analytical techniques, GLP compliance, and a track record of successful publications and awards in the pharmaceutical domain.

🌐 Professional Profiles:

🎓 Academic Background

PhD Scholar: Pursuing from Jadavpur University Master of Pharmacy: Jadavpur University (2019) ,Bachelor of Pharmacy: Jadavpur University (2015) ,Diploma in Pharmacy: Institute of Pharmacy, Kalyani (2009) Various other diplomas and certifications in related medical fields

🏢 Work Experience

BioAnalyst LC-MS/MS and Head of Quality Assurance: Taab BioStudy Services, Jadavpur (July 2023 – Present)Senior BioAnalyst LC-MS/MS (Lab Incharge): Taab BioStudy Services, Jadavpur (Aug 2015 – July 2023)Teaching experience at Bio-equivalence study center, Jadavpur University (2018 – Present) Pharmacist roles in renowned healthcare facilities.

🏅 Achievements

National Award: M.N. DEV Memorial Award for outstanding performance in D.Pharm final exam International Scientist Award: Recognized as the Best Researcher at INSO Award for Engineering, Science, and Medicine Various awards and accolades for published research papers

🔬 Research & Publications

Diverse research projects focused on bioanalytical method development, pharmacokinetic studies, and drug analysis in human plasma Author of numerous research papers in esteemed national and international journals

📚 Skills & Competencies

Proficient in analytical method development for drug determination in tablets and plasma using HPLC, LC-MS/MS, and various analytical software Expertise in GLP maintenance, validation of developed methods, and analysis of pharmacokinetic data Industrial exposures, workshops, and training in pharmaceutical analysis

Top Noted Publication:

Simultaneous determination and quantitation of diosmetin and hesperetin in human plasma by liquid chromatographic mass spectrometry with an application to pharmacokinetic studies Published on 2019/5/1 Cited by 12

A critical insight of modern herbal drugs therapy under the purview of toxicity and authenticity Published on 2018 Cited by 12

Determination and quantitation of benzofuran, Indole and piperazine containing selective serotonin reuptake inhibitor vilazodone hydrochloride in human plasma by LC-ESI-MS/MS … Published on 2021 Cited by 7

LC-MS/MS method development and validation of an antihistaminic, calcium channel blocker, di-phenyl-methyl-piperazine group containing cinnarizine in human plasma with an …Published on 2018 cited by 5

Repeated dose toxicity study in rats of the formulation containing metformin and sitagliptin using microwave assisted acrylamide grafted cassia tora as polymer Published on 2016/6/1 Cited by 4

Analytical method development and validation of trimethylazanium containing anti-leishmanial phospholipid drug miltefosine by liquid chromatography quadruple tandem mass … Published on2016/6/1 Cited by 4

Microwave Assisted Acrylamide Grafting on a Natural Gum Cassia Tora: Characterization and Pharmacokinetic Evaluation of the Formulation Containing Metformin and Sitagliptin in …Published on 2022 Cited by 1

Anti-Hypertensive Activity of Some Selected Unani Formulations: An Evidence-Based Approach for Verification of Traditional Unani Claims Using LC-MS/MS for the Evaluation of … Published on 2022/8/8

Daniele Florio | Pharmaceutical science | Young Scientist Award