Daniele Florio | Pharmaceutical science | Young Scientist Award

Mr. Daniele Florio | Pharmaceutical science | Young Scientist Award

Daniele Florio is a dedicated researcher in pharmaceutical sciences, currently pursuing a Ph.D. at the University of Naples Federico II. His doctoral work focuses on integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia, scheduled for completion in October 2024. Daniele brings extensive experience from his Marie Sklodowska-Curie secondment and voluntary internship, specializing in peptide synthesis, mass spectrometry, and spectroscopic techniques. With a strong publication record and active participation in international conferences, he contributes significantly to the fields of amyloid aggregation, chemical catalysis, and drug development, showcasing advanced skills in scientific research and analysis.

Profile

Scopus

šŸ‘Øā€šŸ«Professional Experience

Tutoring Activities (Grant Type B) 2023-2024, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Provided supplementary teaching for Chemistry and Inorganic Chemistry courses for Pharmacy and CTF degree students. Ph.D. Candidate in Pharmaceutical Sciences (XXXVII Cycle) 2021 ā€“ Present, Naples, Italy, Department of Pharmacy, University of Naples Federico II, PhD Project: Integrated molecular approaches targeting mutated Nucleophosmin 1 in Acute Myeloid Leukemia., Scheduled PhD end: October 31, 2024. Secondee under Marie Skłodowska-Curie Research and Innovation Staff Exchange Action June 2022 ā€“ March 2023, Dublin, Ireland, Keladapharmachem, Participated in the GreenX4Drug (EU funded H2020 RISE project), focusing on developing novel asymmetric chemical catalysts for manufacturing high-value APIs and advanced intermediates.. Voluntary Internship Post-Degree 2018 ā€“ 2019, Naples, Italy, Department of Pharmacy, University of Naples Federico II, Designed, synthesized, and characterized peptides, peptidomimetics, and small molecules using solid-phase and solution methodologies. Utilized mass spectrometry (ESI) for identifying synthetic compounds and applied analytical and preparative methodologies (HPLC) for their purification and characterization. Conducted structural and functional characterization of biomolecules through spectroscopic techniques (CD, Fluorescence, SPR, UV-Vis). Supervisor: Prof. Daniela Marasco

šŸŽ“Education and Training

Biologist Board Examination February 7, 2022, University of Naples Federico II, Naples, Italy Masterā€™s Degree in Biological Science (Molecular Diagnostics Field) July 12, 2021, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Protein fragments of Nucleophosmin 1 as models of amyloids”, Supervisor: Prof. Antonello Merlino, Co-Supervisor: Prof. Daniela Marasco,k Grade: 110/110. Bachelorā€™s Degree in Biological Science (Molecular Diagnostics Field) March 15, 2018, Naples, Italy Department of Pharmacy, University of Naples Federico II Experimental Thesis: “Amyloid-like aggregation of Nucleophosmin 1 regions related to Acute Myeloid Leukemia: spectroscopic studies” Supervisor: Prof. Daniela Marasco Grade: 106/110

šŸ“–Certifications

English TELSA C1, Grade B Language Certification ESAT, Malta Further & Higher Education Authority

Skills and Competencies

Organizational Skills

  • Efficient under pressure.
  • Adaptive to changes and new situations.
  • Strong independent work organization and goal achievement.

Job-related Skills

  • Solid-phase peptide synthesis and combinatorial peptide libraries using Fmoc/Boc chemistry.
  • Experience with various peptide synthesizers (BIOTAGE, Liberty Blue 2.0).
  • Proficient in analysis and purification using ESI mass spectrometry, HPLC, and flash purification systems.
  • Skilled in spectroscopic techniques (CD, Fluorescence, DLS, SPR, UV-Vis, FTIR, NMR).

Digital Skills

  • Proficient in Microsoft Office Suite.
  • Experienced with operating systems: MS-Windows, MacOS, LINUX.
  • Proficient in scientific data analysis software (GraphPad Prism).
  • Knowledgeable in databases (PubMed) and bibliographic management software (EndNote, Mendeley).
  • Proficient with video conferencing software (Teams, Skype, Zoom, Google Meet).
  • Excellent knowledge of major browsers (Edge, Chrome, Firefox, Safari, Opera).

šŸŒProjects

National Project Participation

  • BANDO-PRIN-2022. Research Project: Design, synthesis, and investigation of novel G-quadruplex aptamers against STAT3 and the interleukin-6 receptor (G4-APTASTAT).
  • Project Coordinator and Head of Research Unit: Prof. GALEONE Aldo

Bibliometric Parameters

  • H-index from Scopus: 11
  • Number of Publications: 24
  • Citations: 388

 

Publications Top Notes šŸ“

  • The effects of histidine substitution of aromatic residues on the amyloidogenic properties of the fragment 264ā€“277 of nucleophosmin 1
    • Authors: D. Florio, P. Luciano, C. Di Natale, D. Marasco
    • Journal: Bioorganic Chemistry
    • Volume: 147
    • Year: 2024
    • Pages: 107404

 

  • Could Targeting NPM1c+ Misfolding Be a Promising Strategy for Combating Acute Myeloid Leukemia?
    • Authors: D. Florio, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 25
    • Issue: 2
    • Year: 2024
    • Pages: 811

 

  • Modulation of hydrogel networks by metal ions
    • Authors: S. La Manna, D. Florio, C. Di Natale, D. Marasco
    • Journal: Journal of Peptide Science
    • Volume: 29
    • Issue: 8
    • Year: 2023
    • Article ID: e3474

 

  • Ruthenium complexes bearing glucosyl ligands are able to inhibit the amyloid aggregation of short histidine-peptides
    • Authors: D. Florio, S. La Manna, A. Annunziata, M. Monti, D. Marasco
    • Journal: Dalton Transactions
    • Volume: 52
    • Issue: 25
    • Year: 2023
    • Pages: 8549ā€“8557

 

  • Insights into Network of Hot Spots of Aggregation in Nucleophosmin 1
    • Authors: D. Florio, S. La Manna, C. Di Natale, A.M. Malfitano, D. Marasco
    • Journal: International Journal of Molecular Sciences
    • Volume: 23
    • Issue: 23
    • Year: 2022
    • Pages: 14704

 

  • Hydrogelation tunability of bioinspired short peptides
    • Authors: S. La Manna, D. Florio, V. Panzetta, C. Di Natale, D. Marasco
    • Journal: Soft Matter
    • Volume: 8
    • Year: 2022
    • Pages: 8

 

Abasalt Hosseinzadeh Colagar | Molecular Immunology | Best Researcher Award

Prof. Abasalt Hosseinzadeh Colagar | Molecular Immunology| Best Researcher Award

Prof, University of Mazandaran, Iran

A distinguished molecular biologist with a Ph.D. from Razi University and extensive academic leadership at the University of Mazandaran. Specializing in molecular medicine, genetic diversity, fungal pathogenesis, and plant defenses, I have presented groundbreaking theories and achieved top academic honors. With a track record of pioneering research, I focus on cancer, fertility, and infertility. As Head of the Faculty of Basic Sciences and Nano and Biotechnology Group, I lead innovative research initiatives and mentor future scientists. Committed to advancing biotechnology and molecular biology, I contribute significantly to academia and scientific discourse.šŸŒŸ

Profile

Scopus

Research Interests šŸ§¬

šŸ”¬ Major Field: Molecular Biology and Biotechnology

  • Molecular Medicine: Focus on Cancer, Fertility, and Infertility
  • Molecular Biology: Investigating Genetic Diversity, Molecular Mechanisms of Fungal Pathogenesis, and Plant Defenses
  • Biomedical Engineering: Exploring Plasma Sterilization and Genetic Induction of Microorganisms for Industrial Productions

Educational Records šŸ“š

  • Ph.D. in Molecular and Cell Biology
    Razi University in collaboration with National Institute for Genetic Engineering & Biotechnology (NIGEB), Kermanshah and Tehran, I.R. of Iran
    2000 – 2005

    • GPA: 18.39/20
  • M.Sc. in Molecular and Cell Biology
    Department of Biology, Razi University, Kermanshah, I.R. of Iran
    1997 – 2000

    • GPA: 18.56/20
  • B.Sc. in General Biology
    Department of Biology, Ferdowsi University, Mashhad, I.R. of Iran
    1993 – 1997

    • GPA: 18.24/20

Honors and Awards šŸ†

Presented a Global Theory at California Urology Society, selected for initial publication, offering a method for the professional urology community. Beyond the Abstract, First Iranian Ph.D. graduate in Molecular and Cell Biology in I.R. of Iran, Ranked first among 2000 M.Sc. graduates of Molecular and Cell Biology, Razi University, Kermanshah, I.R. of Iran, Ranked first among 1999 M.Sc. Students in science of Razi University, Kermanshah, I.R. of Iran, Ranked first among 1997 B.Sc. graduates of Biology, Ferdowsi University, Mashhad, I.R. of Iran

 

Academic Experience (Career) šŸ‘©ā€šŸ«

Professor University of Mazandaran August 2014 – Present Head of Faculty of Basic Sciences University of Mazandaran 2010 – 2013 Head of Nano and Biotechnology Group University of Mazandaran 2006 – Present Head of Central Library and Document Center University of Mazandaran 2007 – Present Assistant Professor University of Mazandaran Sep. 2005 – Nov. 2009 Head of Biology Dept. University of Mazandaran 2006 – 2007 Lecturer Teacher Education Center, Kermanshah, I.R. of Iran 1999 – 2000 Lecturer Emam Ali Primary University Center of Kermanshah, I.R. of Iran 1999 – 2000

Publications Top Notes šŸ“

  1. Title: Thymoquinone and its therapeutic potentials
    • Journal: Pharmacological Research
    • Year: 2015
    • Volume: 95
    • Pages: 138-158
    • Citations: 508

 

  1. Title: Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men
    • Journal: Nutrition research
    • Year: 2009
    • Volume: 29
    • Issue: 2
    • Pages: 82-88
    • Citations: 438

 

  1. Title: Zinc is an Essential Element for Male Fertility: A Review of Zn Roles in Menā€™s Health, Germination, Sperm Quality, and Fertilization
    • Journal: Journal of Reproduction & Infertility
    • Year: 2018
    • Volume: 19
    • Issue: 2
    • Pages: 69-79
    • Citations: 316

 

  1. Title: Ascorbic acid in human seminal plasma: determination and its relationship to sperm quality
    • Journal: Journal of Clinical Biochemistry and Nutrition
    • Year: 2009
    • Volume: 45
    • Issue: 2
    • Pages: 144-149
    • Citations: 171

 

  1. Title: Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men
    • Journal: Iranian Red Crescent Medical Journal
    • Year: 2013
    • Volume: 15
    • Issue: 9
    • Pages: 780
    • Citations: 163

 

  1. Title: DNA interaction with Alā€“N, Nā€²-bis (salicylidene) 2, 2ā€²-phenylendiamine complex
    • Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
    • Year: 2007
    • Volume: 67
    • Issue: 2
    • Pages: 133
    • Citations: 133

 

  1. Title: Cigarette smoking and the risk of male infertility
    • Journal: Pakistan Journal of Biological Sciences
    • Year: 2007
    • Volume: 10
    • Issue: 21
    • Pages: 3870-4
    • Citations: 104

 

  1. Title: E. coli, P. aeruginosa, and B. cereus bacteria sterilization using afterglow of non-thermal plasma at atmospheric pressure
    • Journal: Applied Biochemistry and Biotechnology
    • Year: 2010
    • Volume: 160
    • Issue: 7
    • Pages: 1978-1984
    • Citations: 88

 

  1. Title: Association of C677T transition of the human methylenetetrahydrofolate reductase (MTHFR) gene with male infertility
    • Journal: Reproduction, Fertility and Development
    • Year: 2016
    • Volume: 28
    • Issue: 6
    • Pages: 786-794
    • Citations: 71

 

  1. Title: Microsatellite (SSR) amplification by PCR usually led to polymorphic bands: Evidence which shows replication slippage occurs in extend or nascent DNA strands
    • Journal: Molecular Biology Research Communications
    • Year: 2016
    • Volume: 5
    • Issue: 3
    • Pages: 167-174
    • Citations: 60